引用本文: 赵振锐，钟睿，张兴旭. 内生真菌和丛枝菌根真菌互作对醉马草幼苗生长和镉耐性的影响. 草业科学, 2020, 37(3): 432-443 doi: shu

Citation: ZHAO Z R, ZHONG R, ZHANG X X. Effects of interaction of and arbuscular mycorrhizal fungi on the seedling growth and cadmium (Cd) tolerance of . Pratacultural Science, 2020, 37(3): 432-443 doi: shu

内生真菌和丛枝菌根真菌互作对醉马草幼苗生长和镉耐性的影响

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重庆欢乐生肖 lanzhoudaxuecaodinongyeshengtaixitongguojiazhongdianshiyanshi / lanzhoudaxuecaodinongyekejixueyuan，gansu lanzhou 730020

作者简介: 赵振锐(1998-)，男，山西文水人，在读本科生，研究方向为禾草内生真菌学。E-mail: .cn;

通讯作者: 张兴旭, .cn

摘要: 本研究通过接种土壤从枝菌根(arbuscular mycorrhizal, AM)真菌孢子，探讨了在温室盆栽条件下，甘肃内生真菌(Epichloë gansuensis)在不同重金属镉(Cd²⁺)离子胁迫下对醉马草(Achnatherum inebrians)根系AM真菌侵染率的影响，以及内生真菌、Cd²⁺和AM真菌三者互作对醉马草幼苗生长的影响。结果表明，Cd²⁺和内生真菌互作对醉马草菌根侵染率无显著(P > 0.05)影响，菌根侵染率随着Cd²⁺浓度的增加呈现先升后降的趋势，在高浓度Cd²⁺ (0.9 mmol·L^–1)条件下，内生真菌侵染(endophyte-infected, EI)的醉马草植株菌根侵染率显著(P < 0.05)高于不带菌(endophyte-free, EF)的植株。低浓度Cd²⁺ (0.3 mmol·L^–1)显著(P < 0.05)促进了醉马草的生长，而高浓度Cd²⁺ (0.9 mmol·L^–1)条件下显著(P < 0.05)抑制了醉马草的生长；内生真菌在高浓度Cd²⁺ (0.9 mmol·L^–1)胁迫条件下能够减轻其对醉马草的伤害；接种AM真菌显著(P < 0.05)促进醉马草地上部分生长的同时显著(P < 0.05)抑制了其根系的生长；三者互作显著(P < 0.05)促进醉马草叶绿素含量和地上鲜重，但显著(P < 0.05)抑制了醉马草根长。以上结果表明，内生真菌和Cd²⁺均能影响AM真菌对醉马草根系的侵染，但二者无互作效应，内生真菌、AM真菌和Cd²⁺三者互作会影响醉马草幼苗地上和地下生长。

关键词:

English

Effects of interaction of Epichloë gansuensis and arbuscular mycorrhizal fungi on the seedling growth and cadmium (Cd) tolerance of Achnatherum inebrians

state key laboratory of grassland agro-ecosystems / college of pastoral agricultural science and technology, lanzhou university, lanzhou 730020, gansu, china

Corresponding author: Xingxu ZHANG, .cn

Received Date: 2018-06-03
Available Online: 2019-03-01

Abstract: The present study was conducted to measure the colonization rate of arbuscular mycorrhizal (AM) fungi under different growth conditions and to analyze the effects of interaction between Epichloë gansuensis, AM fungi, and Cd²⁺ on the growth of Achnatherum inebrians seedlings. The results indicated that colonization rate of AM fungi increased first and then decreased with the increase of Cd²⁺ concentration. AM fungi colonization rate of endophyte-infected (EI) A. inebrians was significantly (P < 0.05) higher than that of endophyte-free (EF) A. inebrians under a high concentration (0.9 mmol·L^–1) Cd²⁺. However, the interaction of Cd and E. gansuensis had no significant (P > 0.05) effect on AM fungi colonization rate. Low Cd²⁺ concentration (0.3 mmol·L^–1) significantly (P < 0.05) promoted plant growth, whereas high Cd²⁺ concentration (0.9 mmol·L^–1) significantly (P < 0.05) inhibited plant growth. E. gansuensis significantly (P < 0.05) alleviated the damage to host plants under a high Cd²⁺ concentration (0.9 mmol·L^–1). AM fungi significantly (P < 0.05) promoted aboveground growth but significantly (P < 0.05) inhibited belowground growth. The interaction of the three factors significantly (P < 0.05) promoted chlorophyll content and aboveground fresh weight, but significantly inhibited root length (P < 0.05). These results showed that both E. gansuensis and Cd²⁺ affected AM fungi colonization rate, but there was no interaction between them, and the interaction between these three factors affected the growth of A. inebrians seedlings aboveground and belowground.

Key words:

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重庆欢乐生肖

图 1 重庆欢乐生肖土壤样品中检测到的丛枝菌根真菌操作分类单元(种水平)的邻接法进化树

Figure 1. Neighbor-Joining (NJ) tree showing all arbuscular mycorrhizal fungal OTUs/species detected in soil

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图 2 内生真菌和Cd²⁺重庆欢乐生肖互作对醉马草菌根侵染率的影响

Figure 2. Effects of Epichloë endophyte and Cd²⁺ on the mycorrhizal colonization associated with Achnatherum inebrians

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图 3 内生真菌和Cd²⁺重庆欢乐生肖互作对醉马草幼苗株高的影响

Figure 3. Effects of Epichloë endophyte and Cd²⁺重庆欢乐生肖 on the plant height of drunken horse grass

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图 4 内生真菌、AM真菌和Cd²⁺重庆欢乐生肖三者互作对醉马草地上鲜重的影响

Figure 4. Effects of Epichloë endophyte (E), arbuscular mycorrhizal (AM) fungi, and Cd²⁺重庆欢乐生肖 on the aboveground fresh weight of drunken horse grass

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图 5 内生真菌和Cd²⁺互作对醉马草地上干重的影响

Figure 5. Effects of Epichloë endophyte and Cd²⁺重庆欢乐生肖 on the aboveground dry weight of drunken horse grass

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图 6 内生真菌、AM真菌和Cd²⁺三者互作对醉马草叶绿素含量的影响

Figure 6. Effects of Epichloë endophyte (E), arbuscular mycorrhizal (AM) fungi, and Cd²⁺ on the chlorophyll content of drunken horse grass

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图 7 内生真菌、AM真菌和Cd²⁺三者互作对醉马草根长的影响

Figure 7. Effects of Epichloë endophyte (E), arbuscular mycorrhizal (AM) fungi, and Cd²⁺ on root length of drunken horse grass

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图 8 内生真菌、AM真菌和Cd²⁺重庆欢乐生肖三者互作对醉马草根鲜重的影响

Figure 8. Effects of Epichloë endophyte (E), arbuscular mycorrhizal (AM) fungi, and Cd²⁺ on root fresh weight of drunken horse grass

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图 9 内生真菌和Cd²⁺重庆欢乐生肖两者互作对醉马草根干重的影响

Figure 9. Effects of Epichloë endophyte and Cd²⁺重庆欢乐生肖 concentration on root dry weight of drunken horse grass

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表 1 内生真菌、AM真菌和Cd²⁺三者互作对醉马草地上部分生长及生理的影响的三因素方差分析

Table 1. Three-way ANOVA of the effects of Epichloë endophyte (E), arbuscular mycorrhizal (AM) fungi, and Cd²⁺ on the aboveground growth and physiology of drunken horse grass

因素 Factor	株高 Plant height/cm		叶绿素 Chlorophyll content/(mg·dm^–2)		地上鲜重 Aboveground fresh weight/g		地上干重 Aboveground dry weight/g
因素 Factor	F	P	F	P	F	P	F	P
AM	1.329	0.253	307.506	0.000	35.021	0.000	0.002	0.962
E	25.091	0.000	62.654	0.000	14.308	0.000	30.866	0.000
Cd	38.334	0.000	35.644	0.000	32.377	0.000	49.775	0.000
AM × E	0.875	0.353	32.461	0.000	0.819	0.369	0.546	0.463
AM × Cd	0.792	0.503	15.014	0.000	1.898	0.139	1.068	0.369
E × Cd	6.380	0.001	14.746	0.000	2.395	0.076	6.485	0.001
AM × E × Cd	1.368	0.261	10.350	0.000	2.832	0.045	2.076	0.112
AM: 丛枝菌根；E: 内生真菌；Cd: 镉。表2同。　AM: arbuscular mycorrhizal; E: endophyte; Cd: cadmium; similarly for Table 2.

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表 2 内生真菌、AM真菌和Cd²⁺重庆欢乐生肖三者互作对醉马草根生长的影响

Table 2. Effects of Epichloë endophyte (E), arbuscular mycorrhizal (AM) fungi, and Cd²⁺ on root growth of drunken horse grass

因素 Factor	根长 Root length/cm		根鲜重 Root fresh weight/g		根干重 Root dry weight/g
因素 Factor	F	P	F	P	F	P
AM	89.912	0.000	0.069	0.794	5.778	0.019
E	24.970	0.000	6.655	0.012	5.216	0.026
Cd	26.882	0.000	12.989	0.000	17.855	0.000
AM × E	4.424	0.039	25.375	0.000	1.450	0.233
AM × Cd	2.509	0.067	3.535	0.020	0.892	0.450
E × Cd	6.626	0.001	5.311	0.002	5.144	0.003
AM × E × Cd	3.539	0.019	0.920	0.436	0.534	0.661

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shenyanghuagongdaxuecailiaokexueyugongchengxueyuan shenyang 110142

重庆欢乐生肖

内生真菌和丛枝菌根真菌互作对醉马草幼苗生长和镉耐性的影响

作者简介: 赵振锐(1998-)，男，山西文水人，在读本科生，研究方向为禾草内生真菌学。E-mail: .cn;

通讯作者: 张兴旭, .cn

English

Effects of interaction of Epichloë gansuensis and arbuscular mycorrhizal fungi on the seedling growth and cadmium (Cd) tolerance of Achnatherum inebrians

Corresponding author: Xingxu ZHANG, .cn

重庆欢乐生肖

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